Quality control material for plasma fibrinogen test produced from purified human fibrinogen

Quality control material for plasma fibrinogen test produced from purified human fibrinogen

Plasma fibrinogen measurement is a routine laboratory procedure commonly performed on automated coagulation analysers. Its determination is quantitative, not quantitative. Yet, a lack of precision has been an issue for fibrinogen measurement. A control material derived from plasma comprises many proteins, inhibitors and fatty acids, any or all of which can interfere in the fibrinogen assay. Thi...

Proposed fibrinogen standard material with purified fibrinogen for plasma fibrinogen measurement on coagulation analyser

A commercially available fibrinogen standard calibrated by a World Health Organization (WHO) reference material is widely used in Japan, and most clinical laboratories use the Clauss method for plasma fibrinogen measurement. However, a current issue in fibrinogen measurement is poor laboratory-to-laboratory variability. To improve the reliability of fibrinogen values and thereby solve the poor ...

Purification of Fibrinogen from Human Plasma

Studies of platelet fibrinogen from a subject with a congenital plasma fibrinogen abnormality (fibrinogen Paris I).

Fibrinogen from normal platelets and plasma was compared with material derived from a subject with the congenital fibrinogen abnormality termed fibrinogen Paris I. The molecular abnormality in this subject is characterized by the presence (in plasma molecules) of relatively large mutant ‘y-chains (‘y-Paris I) that replace 50% or more of the normal “y-chain population and that, unlike normal ‘y-...

Latex-bound antifibrinogen test for plasma fibrinogen assay.

The semiquantitative latex-bound antifibrinogen test (Fi test) can be modified to quantitate plasma fibrinogen. The test is rapid and simple and does not require complex apparatus. Although the Fi reagent reacts with early fibrinogen and fibrin breakdown products, the degree of interference is not marked and the test is sufficiently accurate to be of clinical value as a fibrinogen assay in pati...